

OPTOSPLIT II & III LS: camera chip is spltted to 2 or 3 channels
Two or three images can be captured simultaneously offering a major benefit over manual or electronic filter changers. A rectangular aperture is used to define the region to be imaged, with a set of simple controls allowing the user to vary the relative positions of the two output images on the camera.
KEY BENEFITS
• Works with sensor sizes up to 22mm diagonal (eg 5.5MPixel sCMOS)
• Dichroic mirror and emission filters mounted in readily interchangeable cubes for dual or triple emission separation
• Optional magnification or demagnification
• Unsplit mode through either channel or neither (18mm diagonal)
• Simple intuitive alignment in x, y, and focal planes
• Interchangeable mount for N.D. filter or chromatic correction lens
• OptoSplit II ByPass - single lever switching from split (dual channels) to bypass (single wavelength recording)
• C-mount coupling to microscope or camera lens
• Standard spectral range from 450 to 900nm
• Supports cropped sensor modes
APPLICATIONS (2 way splitting)
• Ratiometric ion or voltage imaging
• Förster Resonance Energy Transfer (FRET)
• Polarisation studies
• Dual probe widefield microscopy
• TIRF/Spinning disk confocal
• Combined fluorescence/transmitted light microscopy
APPLICATIONS (3 way splitting)
• Triple fluorescence probe studies
• Polarisation Förster Resonance Energy Transfer (pFRET)
• Ratiometric ion imaging
• Polarisation studies
• Simultaneous phase contrast and fluorescence
• Multi-depth imaging


