RMC PTFL
The new PTFL fluorescence location system, developed from a collaboration between the Francis Crick Institute and RMC Boeckeler, is an ultramicrotome-mounted microscope, capable of imaging fluorescent cells in the block during trimming and sectioning. With the PTFL, resin embedded samples can be quickly evaluated and the ROI (region of interest) targeted.
The operator can track the ROI during trimming until the fluorescent cells reach the block surface. The researcher can then collect ultrathin sections of only the ROI for TEM (transmission electron microscopy) using support grids and SEM (scanning electron microscopy) utilizing a substrate of choice, for example, silicon. For Serial Block Face SEM and Focused Ion Beam SEM, the trimmed blockface containing the ROI can then be removed and mounted for volume EM.
Save time: on-the-fly targeting of fluorescent ROIs can save many hours
Save costs: reducing user time, microscope time and data storage
Save storage space: data is only acquired from the ROIs, with big savings in data storage and handling requirements.
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